The salting out procedure outlined in Fig. 5-5 results in significant protein purification by eliminating many less-

Question:

The salting out procedure outlined in Fig. 5-5 results in significant protein purification by eliminating many less- and more-soluble proteins. What is its other advantage?

Fantastic news! We've Found the answer you've been seeking!

Step by Step Answer:

Related Book For book-img-for-question

Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

See More Books

Question Posted: Apr 30, 2016 02:29 PM

See More Questions

Purification of Myoglobin (Mb). Purification tables are often used to keep track of the yield and purification of a protein. The specific activity is a ratio of the amount of the protein of interest,...
The technique of Western blotting can be used to detect proteins that are translated from a particular mRNA. This method is described in Chapter 20 and also in experimental question E5. Let's suppose...
The phosphorylation of a protein can influence its ability to interact with other proteins. These protein-protein interactions play a fundamental role in signal transduction pathways, and these...
In Australia, the banking sector is dominated by four main institutions, the ANZ Banking Group, Commonwealth Bank, National Australia Bank, and Westpac. To maintain a competitive banking market, the...
Michiko and Saul are planning to attend the same university next year. The university estimates tuition, books, fees, and living costs to be $12,000 per year. Michikos father has agreed to give her...
In the Forecast Range section of its annual report MWW provides forecasted statements of earnings and forecasted balance sheets under conservative and optimistic scenarios (see page A-000). Use the...
What is environmental uncertainty, and what are its dimensions? LO.1
During the summer between his junior and senior years, James Cook needed to earn sufficient money for the coming academic year. Unable to obtain a job with a reasonable salary, he decided to try the...
Task 1 (3 Marks) You bought a new house for OMR (student number). The payment terms are 5 years same as cash. If you can earn 12% on your money, how much money should you set aside today in order to...
Consider the following parlor game to be played between two players. Each player begins with three chips: one red, one white, and one blue. Each chip can be used only once. To begin, each player...
You have several cell lysates and want to determine which of them contain protein X in its phosphorylated form (with phosphorylation occurring on a tyrosine residue). You have two antibodies: One...
You wish to separate two proteins in solution by selectively precipitating one of them. What method could you use other than salting out?
In what ways are securities firms and investment banks financial intermediaries?
Pacifico Company, a U . S . - based importer of beer and wine, purchased 1 , 7 0 0 cases of Oktoberfest - style beer from a German supplier for 4 5 9 , 0 0 0 euros. Relevant U . S . dollar exchange...
Consider each of the following scenarios and identify a behavioral intervention to address each issue in family work. A teenager not complying with curfew. One member of the couple not picking up...
Sandy Crane Hospital expanded its maternity ward to add patient rooms for extended hospital stays. They negotiated a 15-year loan with monthly payments and a large sum of $250,000 due at the end of...
2 (39 marks) R QUESTION 2 (39 marks) Roundworm Ltd is a group of companies with a 31 December year-end. The Roundworm group financial statements for the years 20.21 and 20.22 are given below:...
Vino Veritas Company, a U.S.-based importer of wines and spirits, placed an order with a French supplier for 1,400 cases of wine at a price of 240 euros per case. The total purchase price is 336,000...
A quadrillion, 1 x 10 15 , is the approximate number of red blood cells in 50,000 people. Which is greater: a quadrillion red blood cells or the number of nickel atoms in a 5-g nickel coin?
(a) Use integration by parts to show that (b) If f and g are inverse functions and f' is continuous, prove that (c) In the case where f and t are positive functions and b > a > 0, draw a diagram to...
Virus-infected cells produce the intracellular messenger cGAMP, a cyclic dinucleotide in which the GMP 2-OH group is linked to the 5-phosphate of AMP. Draw its structure.
Calculate the contour length for the gene in in Problem 3 if it assumed an A-DNA conformation. Problem 3 Amino acid residues in proteins are each specified by three contiguous bases. What is the...
Gout resulting from the de novo overproduction of purines can be distinguished from gout caused by impaired excretion of uric acid by feeding a patient 15N-labeled glycine and determining the...
On NSE (Indian stock exchange), shares of ICICI Bank trade for 935 rupees. If the spot exchange rate is USD 0.012, what is the no-arbitrage USD price of ICICI Bank ADR? Assume that transactions costs...
Income Statement Balance Sheet Balance Sheet Additional Financial Information 1. Market price of Ranfield's common stock: $90.44 at December 31, 2024, and $58.35 at December 31, 2023. 2. Common...
There is a credit rating agency for businesses that gives out various amounts of information based on the subscription level. This company is called a. Business Credit Scoring b. Fair Issue c. Dun...

The salting out procedure outlined in Fig. 5-5 results in significant protein purification by eliminating many less-

Question:

Step by Step Answer:

Following salting out the precipitated protein can be redissolved in ...View the full answer

Fundamentals of biochemistry Life at the Molecular Level

Students also viewed these Medical Sciences questions