A 50-mL crude extract of skeletal muscle contains 32 mg of protein per mL. TenL of the

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A 50-mL crude extract of skeletal muscle contains 32 mg of protein per mL. TenL of the extract catalyzes a reaction at a rate of 0.14 μmol product formed per minute. The extract was fractionated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in 10 mL. This solution contains 50 mg/mL protein. Ten L of this purified fraction catalyzes the reaction at a rate of 0.65 μmol/min.
(a) What is the degree of purification (fold purification)?
(b) What is the percent yield of the enzyme recovered in the purified fraction?
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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