A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm.
Question:
(a) Calculate the volume, Vt, of the column, which is equal to πr2l.
(b) The void volume, Vo, was 18.1 mL, and the total volume of mobile phase was 35.8 mL. Find Kav for a solute eluted at 27.4 mL.
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