(a) How does the amperometric glucose monitor in Figure 16-10 work?

(b) Why is a mediator advantageous in the glucose monitor?

(c) How does the coulometric glucose monitor in Figure 16-12 work?

(d) Why does the signal in the amperometric measurement depend on the temperature of the blood sample, whereas the signal in coulometry is independent of temperature? Do you expect the signal to increase or decrease with increasing temperature in amperometry?

(e) Glucose (C₆H₆O₁₂, FM 180.16) is present in normal human blood at a concentration near 1 g/L. How many microcoulombs are required for complete oxidation of glucose in 0.300 μL of blood in a home glucose monitor if the concentration is 1.00 g/L?

Figure 16-10

Figure 16-12

Transcribed Image Text:

Test strip Ag I AgCI reference electrode Carbon working electrode 1 coated with glucose oxidase and mediator Blood is ctd applied here Electrical contacts Carbon working electrode 2 coated with mediator but no enzyme Hydrophilic mesh wicks blood over the electrodes (a) (b)