Mouse genomic DNA is treated with a restriction endonuclease and electrophoresed in an agarose gel. A radioactive

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Mouse genomic DNA is treated with a restriction endonuclease and electrophoresed in an agarose gel. A radioactive probe made from the human gene rxr-1 is used to perform a Southern blot. The experiment was repeated three times. Explain the results of these repeated experiments:
Experiment 1. The autoradiogram shows a large smudge at a position corresponding to the top of the lane in which the mouse DNA was electrophoresed.
Experiment 2. The autoradiogram shows a smudge over the entire lane containing the mouse DNA.
Experiment 3. The autoradiogram shows three bands of varying intensity.
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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