Consider the product concept in Figure 25.13 for the high throughput screening of kinase inhibitors. Two competitive concepts are proposed:

(a) Use no bar codes; that is, use sequential generation of both the kinase enzymes and inhibitors.

(b) Use bar codes for both the kinase enzymes and inhibitors. Determine the number of colors and concentrations necessary to provide bar codes for 10,000 kinase inhibitors.

Estimate the operation times and compare the purchase costs and net present values of the three concepts over three years.

Figure 25.13:-

Transcribed Image Text:

Well Plates Diluent (pH buffer, salt,...) KE,....KE Peptide JANUS Robot 3.15 sec/KE Robot Robot TransFluoSpheres (fluorescent beads) for Bar Codes 3 Colors, 3 Conc. RainDance PLS Chip ECHO 555TM Liquid Handler Forms KE Mixtures Robot KE/Pop.....KE/Pep Emulsion Gun 20,000 drop/sec Kinase Library Robot Microcentrifuge Tube Emulsion Gun 1,000 drop/sec RainDance PLS Chip Robot ATP/ Transcreener Assay Solution Well Plates KIKI JANUS Robot 1.5 sec/oper. Diluent (pH buffer, salt,...) Robot Robot Emulsion Gun Emulsion Gun Emulsion Gun 167 drop/sec 833 drop/sec Combine Droplets 1,0XX0 drop/sec Combine Droplets Complete 2/3 V 1/3 V KEKE Droplets Splitter 1,000 drop/sec Combine Droplets 1,000 drop/sec Capillary Alignment Required 58 slide/hr Placed in CYTOMAT 6000 Stared at 4C 467 Glass Slides 1"x3" Read [ADP] and Bar Codes Robot Incubation CYTOMAT 44 Heated at 37C for 1 hr 4 Agilent Robot SureScan Microarray Readers Figure 25.13 Schematic of operations on and off RainDance PLS chip. Computer Database