Nitrite is commonly determined by a colorimetric procedure using a reaction called the Griess reaction. In this reaction, the sample containing nitrite is reacted with sulfanilimide and N-(1-Napthyl) ethylenediamine to form a colored species that absorbs at 550 nm. Using an automated flow analysis instrument, the following results were obtained for standard solutions of nitrite and for a sample containing an unknown amount:

(a) Find the slope, intercept, and standard deviation of the calibration curve.
(b) Construct the calibration curve.
(c) Determine the concentration of nitrite in the sample and its standard deviation.

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Solution Absorbance at 550 nm 2.00 μΜ 6.00 μΜ 10.00 µM 14.00 µM 18.00 µM Unknown 0.065 0.205 0.338 0.474 0.598 0.402