Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to

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Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.

Data from Figure 3-24:

Xmnl Scal Pvul- Avall Mstl amp Avall BglI Sspl -2000 AatII EcoO109 0 pUC18 (2.69 kb) HgiEII Ndel, HgiEII Narl

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Fundamentals Of Biochemistry Life At The Molecular Level

ISBN: 9781118918401

5th Edition

Authors: Donald Voet, Judith G Voet, Charlotte W Pratt

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