a. The interstitial volume, Vo,Vo, in Figure 26-14 is the volume at which the curves rise vertically at the left. Find VoVo for the 25-nm-pore-size 25-nm-pore-size column. To the nearest order of magnitude, what is the smallest molecular mass of molecules excluded from this column?
b.  What is the molecular mass of molecules eluted at 9.7 mL 9.7 mL from the 12.5-nm 12.5-nm column?
c. VMV_M is the volume at which the curves drop vertically at the right. Find the largest molecular mass that can freely enter the 45-nm 45-nm pores.

Figure 26-14

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Molecular mass (Da) 106 105 104 10³ 10² I Pore size A 12.5 nm 25 nm 45 nm 8 10 Elution volume (mL) Harris/Lucy, Quantitative Chemical Analysis, 10e, 2020 W. H. Freeman and Company 6 12 FIGURE 26-14 Molecular mass calibration graph for hydrophilic silica size exclusion columns (30x0.78 cm) (30 x 0.78 cm) of different pore size. Calibration based on biomolecules ranging from thyroglobulin (660 000 Da) (660 000 Da) to glycine tetramer (246 Da) (246 Da).