Spectrophotometry with indicators. Acid-base indicators are themselves acids or bases. Consider an indicator, HIn, which dissociates according
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Spectrophotometry with indicators.† Acid-base indicators are themselves acids or bases. Consider an indicator, HIn, which dissociates according to the equation
The molar absorptivity, ε, is 2 080 M-1 cm-1 for HIn and 14 200 M-1 cm-1 for In-, at a wavelength of 440 nm.
(a) Write an expression for the absorbance of a solution containing HIn at a concentration [HIn] and In- at a concentration [In-] in a cell of pathlength 1.00 cm. The total absorbance is the sum of absorbances of each component.
(b) A solution containing indicator at a formal concentration of 1.84 × 10-4 M is adjusted to pH 6.23 and found to exhibit an absorbance of 0.868 at 440 nm. Calculate pKa for this indicator.
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a The absorbance of a solution can be calculated using the BeerLambert law A b c where A is the abso...View the full answer
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