1. Consider the following instrument configuration: A multiwavelength, collimated Laser Diode with primary emission at 490 nm (100 mW) is used as a light source. The Laser is focused onto bandpass filter with the transmission spectrum shown in Figure 2. The transmitted light is then directed via a series of mirrors onto a standard 1 cm cuvette similar to the configuration shown in Figure 1. Relevant pieces of info are given below. a. Based on the spectrum of the filter in Figure 2, is this a Fabry-Perot Filter or an absorption filter? Why? b. What is the intensity at point C if the sample cuvette is constructed from F2 glass and two Ag mirrors are used (see reflectance curve below)? Note: values for normal incidence and 45 incidence are the same for this mirror O. D Detector 351.1 363.8 404.7 435.8 441.6 457.9 465.8 472.7 476.5 C. For an absorbance measurement, at what point (a through d) should I be measured? Why? d. If the sample cell contains a non-absorbing species, what is the intensity of light measured at point D if a 1.0 cm SF11 sample cell and Ag mirrors are used in the system? 480.0 486.1 488.0 496.5 501.7 514.5 532.0 546.1 587.6 e. 589.3 632.8 Figure 1 -Instrument schematic Refractive Index of Five Schott Glass Types Wavelength A (nm) BK7 (N-BK7) 1.53894 1.53649 1.53024 1.52668 1.52611 1.52461 1.52395 1.52339 1.52309 1.52283 1.52238 If the SF11 sample cell contains a solution of molecule X at a concentration of 50.0 M ( = 10,000 M cm) what is the intensity of light measured at point D? 1.52224 1.52165 1.52130 1.52049 1.51947 1.51872 1.51680 Source 1.51673 1.51509 Cuvette C SF11 BP-F 1.84208 1.82518 1.82259 1.81596 1.81307 1.81070 1.80946 1.80834 1.80645 1.80590 1.80347 1.80205 1.79880 1.79479 1.79190 1.78472 1.78446 1.77862 B Refractive Index, LaSFN9 - 1.89844 1.88467 1.88253 1.87700 1.87458 1.87259 1.87153 1.87059 1.86899 1.86852 1.86645 1.86524 1.86245 1.85901 1.85651 1.85025 1.85002 1.84489 M1 M2 BaK1 (N-BJK1) 1.60062 1.59744 1.58941 1.58488 1.58415 1.58226 1.58141 1.58071 1.58034 1.58000 1.57943 1.57927 1.57852 1.57809 1.57707 1.57580 1.57487 1.57250 1.57241 1.57041 F2 1.67359 1.66682 1.65064 1.64202 1.64067 1.63718 1.63564 1.63437 1.63370 1.63310 1,63208 1.63178 1.63046 1.62969 1.62790 1.62569 1.62408 1.62004 1.61989 1.61656 % Transmission 100 PERCENT REFLECTANCE 100 80- 95 60 40- 20- 0 480 Figure 2. Transmission spectrum of bandpass filter. MF497-16 486 490 495 500 505 510 515 Wavelength (nm) normal incidence typical raffoctance ou 400 450 500 550 600 650 700 WAVELENGTH IN NANOMETERS reflectance curve for Ag mirror