1. Fasting normal blood glucose concentration is between 70-100 mg % (mg per 100 mL blood). Express this concentration range of glucose in mM.

b. How many mL of 0.2 M sodium acetate should be added to 200 mL of 0.2 M acetic acid to make a buffer of pH 6.5? (refer to Table 2-2 on page 60 for pKa values).

What is the molarity of the resulting buffer with respect to acetate (acetate + acetic acid)?

7. A) How many mL of 0.2 M HCl should be added to 100 mL of 0.2 M Tris (Trishydroxymethyl aminomethane) to make a buffer of pH 7.5? Hint 1: In this case, HCl is not the conjugate acid of Tris. In water, there will be an equilibrium established between Tris base ([R-]) and Tris acid ([RH]) with a pKa of 8.08. 1 mole HCl will be needed to convert 1 mole of Tris base (R-) to Tris acid (RH). Hint 2: working with ratios of mmoles of Tris base, Tris acid, and of needed HCl will help solve this one.