1. Inhibited Michaelis-Menten kinetics ( 30 points): Determine the KM and VM values for an enzyme system based upon the enzyme rate (R) versus substrate concentration [S] data provided in the graph below (this is the enzyme saturation curve). Figure 1 presents the curve for the free reaction (no inhibitor present) while Figure 2 presents the inhibited reaction. Fill in the blanks in all columns in the table on this page and the next page by picking points off of the given curves, and then prepare a double-reciprocal plot showing the lines for both the free reaction and the inhibited reaction using the blank graph (label graph axes). Also, calculate the KM and VM values for both the free reaction and the inhibited reaction from the information in the graph you created. You must show how you finally determine or calculate the KM and VM values for full credit. Figure 1: Free reaction (no inhibitor) saturation curve Table 1: Table of values for free reaction GRAPH FOR DOUBLE RECIPROCAL PLOT (Prob \#1) Figure 2: inhibited reaction saturation curve Free reaction results KM= (include units) Table 2: Table of volues for inhibited reaction VM= (include units) Inhibited reaction results KM= (include units) VM= (include units) 1. Inhibited Michaelis-Menten kinetics ( 30 points): Determine the KM and VM values for an enzyme system based upon the enzyme rate (R) versus substrate concentration [S] data provided in the graph below (this is the enzyme saturation curve). Figure 1 presents the curve for the free reaction (no inhibitor present) while Figure 2 presents the inhibited reaction. Fill in the blanks in all columns in the table on this page and the next page by picking points off of the given curves, and then prepare a double-reciprocal plot showing the lines for both the free reaction and the inhibited reaction using the blank graph (label graph axes). Also, calculate the KM and VM values for both the free reaction and the inhibited reaction from the information in the graph you created. You must show how you finally determine or calculate the KM and VM values for full credit. Figure 1: Free reaction (no inhibitor) saturation curve Table 1: Table of values for free reaction GRAPH FOR DOUBLE RECIPROCAL PLOT (Prob \#1) Figure 2: inhibited reaction saturation curve Free reaction results KM= (include units) Table 2: Table of volues for inhibited reaction VM= (include units) Inhibited reaction results KM= (include units) VM= (include units)