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6. If inhibitor X changes lactase activity to a V0 of 0.111111111111mM per minute when [S]=0.40 mM, and a V0 of 0.166666666667mM per minute when

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6. If inhibitor X changes lactase activity to a V0 of 0.111111111111mM per minute when [S]=0.40 mM, and a V0 of 0.166666666667mM per minute when [S]=1.0mM, calculate the new Vmax. A. 0.10mM per minute B. 0.25mM per minute C. 0.50mM per minute D. 1.0mM per minute E. 2.0mM per minute 7. Calculate the new Km of the lactase reaction with inhibitor X. A. 0.10mM B. 0.25mM C. 0.50mM D. 1.0mM E. 2.0mM 8. Calculate the slope on a Lineweaver-Burk plot (Km/Vmax) for the lactase reaction with inhibitor X A. 0.10 per minute B. 0.25 per minute C. 0.50 per minute D. 1.0 per minute E. 2.0 per minute 9. Inhibitor X exerts which of the following effects on the above enzyme (lactase)? A. uncompetitive inhibition B. mixed noncompetitive inhibition C. competitive inhibition D. pure noncompetitive inhibition E. all of the above

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