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Carbonate-bicarbonate buffer is extensively used in molecular and cell biology, biochemistry and in the medical field. It can be prepared by mixing aqueous solutions

 

Carbonate-bicarbonate buffer is extensively used in molecular and cell biology, biochemistry and in the medical field. It can be prepared by mixing aqueous solutions of Na2CO3 and NaHCO3. CO2 (aq) + H2O (1) HCO3 (aq) + OH (aq) (a) Write a thermochemical equation for the formation of NaHCO3(aq). Kb = 2.14 x 104 (2 marks) (b) Identify TWO conjugate acid-base pair in the above ionization reaction of CO2 (aq). (2 marks) (c) 100.0 cm carbonate-bicarbonate buffer was prepared by dissolving 6.303 g Na2CO3 and 3.405 g NaHCO; in water. Calculate the pH of the carbonate-bicarbonate buffer. (6 marks) (d) If 10.0 cm of 0.100 M HCI is added into the buffer prepared in (c), calculate the new pH. (8 marks)

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