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Azo dye is a synthetic colorant used in food industries to enhance food visual. However, chromophoric group in azo dye is suspected carcinogenic under certain conditions. Several methods including solvent extraction and chromatographic techniques are used to monitor and determine the azo dye content in food products. a. If 50% of azo dye is extracted from 100 ml organic solution using 50 ml sodium chloride solution; i. What is the distribution coefficient, Ko, of azo dye extraction system? [3 Marks] ii. How many percent of azo dye extracted after TWO (2) times extractions with 50 ml of sodium chloride? [3 Marks] iii. Calculate the percentage of unextracted azo dye after THREE (3) times extractions with 50 ml of sodium chloride? [3 Marks] iv. What are the important characteristics of the solvent that must be ascertained before the solvent extraction method is carried out? [2 Marks]