Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (use graph paper from the lab manual, DO NOT PLOT USING EXCEL- if you plot using Excel, 10pts will be deducted!). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s).

SAMPLE	PROTEIN CONCENTRATION	ABSORBANCE, 610 nm
Standard 1	1 mg/ml	0.064
Standard 2	2.5 mg/ml	0.154
Standard 3	5 mg/ml	0.304
Standard 4	7.5 mg/ml	0.449
Standard 5	10 mg/ml	0.613
Unknown A		0.850
Unknown B		0.475