Secrets of the Rain Forest Lesson 8 Marketing-Advocacy Groups and Viewpoints Consider the followiag groupr and bew deir opinions might intluence your eflorts wa ger. your green proccio to the makk: Phase I Identifying the Players For each of the groups below, desente their goals abs bow they might respons to your cancer-curiag procein resecirch. Who are they and what do hicy want? Explain in your own words. You may noed to do some rosesch. Try the inernet or the ressemes in your library, A. FDA (food and Drug Aulminituration) 8. Ramoe asd the otber stomach cancer vicing C. Matthew and other animal activiss from PETA (People for the Ethical Troatuent of Animals) D. Tista and other employees of Biotes Secrets of the Rain Forest Lesson 1 The Mysterious Green Fluorescent Leaves Tisha was hiking through the Andean Rain Forest looking for unique plant species when she met a young boy named Ramon who told her about an old medicine man with mysterious leaves. Ramon said that his own sister had once suffered stomach pains and been diagnosed by a town doctor as having stomach cancer, a painful and usually fatal disease. Ramon's family could not afford the doctor's treatments, so they approached the medicine man who made a small cut in Ramon's sister's arm and placed his mysterious leaves on it. A few months later, when Ramon's sister was examined by the town doctor, she was told that her cancer was cured. Ramon went back to thank the old medicine man and found him very ill. Soon, the old man died and, with him, the secret of the 'mysterious leaves'. Ramon gave Tisha a bottle with a few of the mysterious leaves. Tisha returned to Biotex, a biotechnology company working on developing new medicines to treat human disease. At Biotex, Tisha noticed that the mysterious leaves glowed bright green under her ultraviolet light. Other Biotex scientists determined that the shiny green substance in the leaves was a protein which caused special effects. To make more of this protein, DNA from the leaves was removed and inserted into bacterial cells. Some of these bacteria now contain the gene for the special green protein. Biotex is taking a gamble on developing a cure for stomach cancer. It is a long shot, costing over $100 million dollars in testing over about 10 years, but the potential for curing the disease and making a profit is good. Right now there is enough money in the bank to pay Tisha and the other employees at Biotex for a few more years, but Biotex urgently needs a product so it can survive beyond that. On top of all this, Tisha has received a desperate letter from Ramon. His recurrent stomach aches have been diagnosed as stomach cancer. Appendix A Glossary of Terms Mear Aratrinese Mar terual Libragt A mate roud mi foul by Bakeriat Mexirrial Lasate a tiryle sounc. ln this casey be Dos A came fram the spoies of ples with der argistires poten leirech. Bhotrehnulest Matrmul mleaied tran inaide a byood bacterial cell Chrumatesraply t 7ering A perersa his eparating indminises of comples mas: fares. A (tequafi isianure is poured fturoupt a column parlind o ith siny heals. The properties of the beats pans firish icaby ilereical is try will oretain identisal DNh. The Coluny wally alential they are clane: Ceatrifugation Spineise a mature at hath spowi ia iepuraic bicavy and Truper uaer" that sosides atwene the pellet. Calfure Media made froes ant cuisact of yeits and meat bypcoducts. whinh fruvides natricets for bacterial prumeth. Agar. whind is vary sistilae so len.0, peovider a volid sispor? on stisch lo culte the baxticria. [Tibis) by deter anin g of a hach food ce thaz feally works abs be mated ayn ner ollimm. Grastic Faginetring The nomelucion of att ispanisen's DW A by introducing or che - auring sgoctefe parti of it: Lysouyme Entytne which can tyse, or kreak opon, bacteria cell wails. Pellet Afier enedif pation, the beavier parieles vech as bacInta or the detris of lysed bacteris ane found at the bocbu of a nicrolage tabe in a pellet. Pasmid A circuler DNA molecule, rapoble of indepeudent reptieaticen semstises caryying yenes for antitiotic reis. tunce- pGi.0 Pinmid cankining the GFP Erae under arabione con: trot Aho ceetains a gine for antibiofic resialance. Process of ideneifying desired bucteria froes a bucteral litiniry. Sterile Technigue Minitising the possibility of outside baciertal contamitucive daring an experimcnt by uaine caneful techingque. Sere Aproendix CF. Stroulaing: Process of pasing an iniculating loop with bacteria ce it actoos an agir plute in conler 10 scparate the celle. Superatant Liquit, coessiaing cell ingredients, which ase lighast than the pellet foemed after centrifugatiot. Vectar An indepesdently roplicating DNA molecrile (cj, a plasmidi into which foreign DN fragmenes can be inserthed Sor propugabion: Secrets of the Rain Forest Lesson 9 Name Revlew Questions Marketing-Ethies and Economics Given the financial sitmation of Biotes and wtat the exment market will bear, answer gaestions 13. 1. Which type of cincer, fueg or stomact, will receive the moit fin meial soppeet from Medheare? 2. Which tyre of cancer, lung or stostrich, will eakle Biotex to make the larpeat grofir? Euphin your answer. 3. If Biotex were bo emarict the groes preecin iar boxh tyjes of cuscer, at what price shoulut the company sell the modicatice. the sase poice foc both, or different prices? Explain your atsisct