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The following proteins listed with their molecular weights are separated by gel filtration: Myoglobin (17,000 Daltons), serum albumin (69,000 Dalton), cytochrome c (13,400 Dalton). Which

The following proteins listed with their molecular weights are separated by gel filtration: Myoglobin (17,000 Daltons), serum albumin (69,000 Dalton), cytochrome c (13,400 Dalton). Which protein is expected to be eluted from the column first? What would be the order of migration of these proteins on SDS-PAGE from the top to the bottom of the gel? Briefly explain how each technique works.

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