1. Calculate change in absorbance Abs (0 mins) -Abs (5, 10, 15, OR 20 mins) 2. For tubes 1-4, plot change in absorbance (y-axis) versus elapsed time (x axis) for each reaction. Include the origin as a point for all curves, i.e., the 0 -min value for AA is 0. Draw a smooth curve through the data points for each reaction. Note: The units for time should be the same for all readings (i.e., use either seconds or minutes). What volume of mitochondrial suspension gives the highest initial velocity? 3. Calculate the initial velocity (vo) for each reaction as the slope of a straight line drawn through the first two or three data points. Initial velocity should be expressed as: A absorbance / mins. tubo 1 2 3 4 5 6 7 8 0 mins 5 mins 10 mins 15 mins 20 mins 0.05 0.03 0.07 0.06 0.06 0.01 0.03 0.03 0.08 0.08 0.645 0.486 0.428 0.394 0.378 0.626 0.595 0.387 0.385 0.652 0.526 0.416 0.338 0.734 0.581 0.426 0.334 0.699 0.493 0.308 0.211 0.673 0.478 0.307 0.233 0.621 0.608 0.594 0.586 0.639 0.616 0.576 0.568 0.621 0.620 0.621 0.619 0.632 0.621 0.611 0.601 0.631 0.621 0.620 0.621 0.651 0.621 0.628 0.615 0.661 0.654 0.630 0.621 0.681 0.641 0.621 0.618 0.366 0.268 0.220 0.102 0.109 0.581 0.556 0.611 0.602 0.619 0.611 0.612 0.625