You decide to perform ion exchange chromatography next. You decide to determine actual pl of endonuclease by performing isoelectric focusing. This is an analytical technique - you cannot use it to purify your protein, but you can use it to estimate its pl. Assume that the IEF gel shown to the right has a linear pH gradient from pH = 3 to pH = 9. You slice the gel into different fractions and assay each for nuclease activity. The band marked with * scored positive for this activity. pH 9 OOJ) ) Decreasing pH pH 3 If you perform the ion exchange chromatography at pH = 6.5, would you want to use an anion or cation exchanger? anion exchanger cation exchanger neither, as the protein clearly has no charge Based on the results in the previous question, which would you choose as the best way to elute the protein from the ion exchange column? decrease pH to 4 increase [KC] s decrease [KC] increase pH to 9 none of the above (didn't I already say the protein had no charge??)