Cytochrome C(*p = 0:38) and ovalbumin (* p = 0:34) in an equimolar mixture are to be purified by size exclusion to a resolution of

Cytochrome C(ϵ*p = 0:38) and ovalbumin (ϵ*_p = 0:34) in an equimolar mixture are to be purified by size exclusion to a resolution of R = 1.0 in a packed bed of Toyopearl1 (TSK gel) HW55F, which has a bed porosity of ϵ_b = 0:34. The differential migration velocity, d, for these two solutes is 0.05. Assume a volumetric feed stream/fluid-phase dilution in counter flow of 10. Determine the relative number of equilibrium stages, the relative solvent requirement, and the relative solid-phase requirement for steady counterflow separation relative to differential chromatography.