When the hydration of fumarate is catalyzed by the enzyme fumarase in D2O, only (2s,3R) 3-deuteriomalate is
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When the hydration of fumarate is catalyzed by the enzyme fumarase in D2O, only (2s,3R) 3-deuteriomalate is formed as the product. (Each -CO, group is the conjugate base of a carboxylic acid group.)
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со: + Do fumCH OD CH-D со CO5 3-deuteriomalate (the 2S,3R stereoisomer is formed exclusively) fumarate HO H O.C CO7 D H
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