A lac operon containing one mutation was cloned into a plasmid, which was introduced by transformation into

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A lac operon containing one mutation was cloned into a plasmid, which was introduced by transformation into a bacterium containing a wild-type lac operon. The three genes of the chromosomal operon were rendered non-inducible in the presence of the plasmid. 

(a) What kind of mutation in the plasmid operon could have this effect?

(b) Suppose the result of transformation was to cause the three plasmid lac genes to be expressed constitutively, at a high level. What type of plasmid gene mutation could have this result?

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Biochemistry Concepts And Connections

ISBN: 9780134641621

2nd Edition

Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews

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