In studying the mechanism of a particular enzyme, for which the cloned gene is available, you wish
Question:
In studying the mechanism of a particular enzyme, for which the cloned gene is available, you wish to change a putative active site histidine residue to a proline by site-directed mutagenesis. The His codon to be changed is 5′-CAC. You wish to change it to 5′-CCC, one of four Pro codons. The nucleotide sequence surrounding the His codon is 5′-CTGGAATCTCACTTTATCTGG-3′. Write the nucleotide sequence of an oligonucleotide (a 21-mer) that could force the conversion of the CAC codon to CCC in a site-directed mutagenesis operation.
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Related Book For 
Biochemistry Concepts And Connections
ISBN: 9780134641621
2nd Edition
Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews
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