3) Number of Polypeptide Chains in a Multisubunit Protein A researcher treated a sample (660 mg) of an oligomeric protein with a molecular weight of 132kDa with an excess of 1-fluoro-2,4dinitrobenzene (FDNB aka Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. She then completely hydrolyzed the peptide bonds of the protein by heating it with concentrated HCl. The hydrolysate was found to contain 5.5mg of the compound shown. (1.5 points total) 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. In 2 sentences or less, explain how this information can be used to determine the number of polypeptide chains in an oligomeric protein. Then calculate the number of polypeptide chains in this protein. B) What other analytic technique could you employ to determine whether the polypeptide chains in this protein are similar or different