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The enzyme alkaline phosphatase catalyses the conversion of -glycerophosphate to glycerol and inorganic phosphate (P i ). To characterise alkaline phosphatase isolated from a new

The enzyme alkaline phosphatase catalyses the conversion of -glycerophosphate to glycerol and inorganic phosphate (Pi).

To characterise alkaline phosphatase isolated from a new organism, a 10 minute reaction using 0.5 ml of enzyme sample, and -glycerophosphate substrate was carried out at 37oC. The inorganic phosphate (Pi) released during the reaction was then measured by absorbance in an assay for Pi. It was determined using a standard curve for Pi that 6 mol of Pi was released during the 10 minute reaction.

Which of the following values is correct for the activity of alkaline phosphatase in the enzyme sample, expressed as mol of Piproduced per minute per ml of enzyme?

  1. 6.0
  2. 12.0
  3. 0.12
  4. 1.2

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