Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies Its buffering range is from pH 7 to 9, and Kb is 1 19 Atilde 10 6 for the reaction a What is the optimum pH for TRIS buffers b Calculate the ratio TRIS TRISH at pH 7 00 and at pH 9 00 c...

a The optimum pH for a buffer is when pH pK a At this pH a buffer will have equal neutrali ...

Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering

Question:

Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is from pH 7 to 9, and Kb is 1.19 Ã— 10-6 for the reaction

a. What is the optimum pH for TRIS buffers?
b. Calculate the ratio [TRIS]/[TRISH+] at pH = 7.00 and at pH = 9.00.
c. A buffer is prepared by diluting 50.0 g of TRIS base and 65.0 g of TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer?