When a mixture of the amino acids Phe and Gly is subjected to chromatography in a pH
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When a mixture of the amino acids Phe and Gly is subjected to chromatography in a pH 6 buffer on the ion-exchange resin shown in Eq. 27.9, the Phe emerges from the column much later than the Gly, even though the two amino acids have the same isoelectric point. Explain.
Transcribed Image Text:
-CH₂-CH-CH₂-CH-CH₂-CH-... 0=S=0 0- 0=S=O structure of sulfonated polystyrene with ionized sulfonic acid groups (27.9)
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The resin contains a large number of pendant phenyl groups Phenylalanine also contains a phenyl grou...View the full answer
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